BT220 Steam Rapid

£295.00 + VAT

SKU: BT220 Category:

Description

BT220 STEAM RAPID

Rapid readout fluorescence system. Self-contained biological indicator for  or  sterilization processes

Spore: . in a  or  population.
Applicable regulations: .
Incubation conditions: . Final reading: . Units per box: .

Composition

Each tube contains a population of Geobacillus stearothermophilus ATCC 7953 spores soaked on a filter paper strip (spores carrier). It also has purple colored culture medium contained within the glass ampoule, over the filter next to the carrier, on the base of the tube.
Product description Bionova® BT220 Rapid Readout Fluorescence System Biological Indicators for Steam sterilization processes have been designed for quick and easy monitoring of vacuum-assisted and gravity displacement steam sterilization cycles at 121-135 ºC. They consist of a plastic tube, a special filter, a spores carrier and a glass ampoule with culture medium. The tube has a plastic cap with holes and a barrier permeable to steam.

Rapid readout: 3 hours

The rapid readout must be carried out in the Bionova® IC10/20FR, Bionova® IC10/20FRLCD o Bionova® MiniBio Reader Incubator or in a similar reader incubator that meets the features that are described next.
The Reader-incubator must be capable of reading the fluorescence emission of the product, resulting from breaking a specific substrate on the spores carrier. Fluorescence is produced when the reader stimulates the carrier with UV light at 360 nm. Final readout of negative results is readily available after 3 hours of incubation. Fluorescence is a direct measure of the germination and growth of Geobacillus stearothermophilus spores which have survived the sterilization process (positive result). Furthermore, a failure in the sterilization process can also become evident by the color change of the medium. Due to the high sensitivity of the fluorescence results at 3 hours, conventional incubation for color change of BT220 Rapid Readout Fluorescence System Indicator is not an advantage.

Characteristics

  • Polypropylene random copolymer tube: 50.0 mm high x 8.5 mm
    external diameter. Wall thickness: 0.5 mm
    Polypropylene microfiber cap at the bottom of the tube.
  • Polypropylene cap: 10.5 mm external diameter, 16.5 mm high. Wall
    thickness: 1.0 mm
  • Glass ampoule: 35.0 – 38.0 mm high. mm. External diameter: 6.5 mm.
  • Wall thickness: 0.2 – 0.3 mm. Culture medium 0.5 – 0.7 ml, purple color.
  • Cap filter: medical grade paper, 60 g/m2, 16.0 mm diameter.
    Spores carrier: Filter Paper: 5.0 x 20.0 mm, 160 g/m2.? 106 Geobacillus stearothermophilus ATCC 7953 spores per vial.
    Final fluorescence reading is performed after 3 hour-incubation at 60
    ºC (sensitivity: 97 %).
    An optional visual pH color change confirmation could be made after
    48 hours of incubation. If sterilization process has not been
    successful, culture medium will change to a greenish color first, and
    then to yellow during incubation at 60 ºC, thus showing the presence
    of living spores. If sterilization process is successful culture medium
    will remain purple after the incubation process.
    7-day readout is optional and not intended to be routinely performed; it
    is an initial validation of the 3 hour-reading. Fluorescence results may
    be compared to the 7-day visual reading.
  • NOTE: If 7-day readout is performed, a humidified environment will be
    required to avoid medium to dry out.
  • D-Value: Not lower than 1.5 minutes at 121 ºC. Another D-value is
    informed at 135ºC.
  • Z-Value: Not lower than 6 ºC
Product Description Direction of Use

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