Hydrogen Peroxide Decontamination – Pre-Decon Service

Part of the preparation of new buildings designed for the research in the animal care sector is the pre-decontamination of any lab space before the arrival of the animals to ensure maximum safety from any microorganism introduced into the facility during the construction. In this case study, Sychem's skilled team of specialists provided a fill Hydrogen Peroxide decontamination service on the entire perimeter of the lab unit within a larger research building. To help to understand how the decontamination process works, Sychem's Engineer and decontamination Specialist answers some questions about the method and validation of the operation:

What is the preparation required before the Hydrogen peroxide Decontamination vapours (VPHP) is released? For the full decontamination and validation of the operation in a large size unit, the set up of indicators, sensors, and generators are key. Five Bioreset Smart generators were positioned in the main corridors of the designed area to ensure the Hydrogen Peroxide vapour reaches all the areas of the room.

What are the prerequisites needed to achieve maximum decontamination? The crucial conditions required to achieve decontamination are mainly a. Temperature in the rooms and b. the Level of Humidity in the air. The temperature in the rooms should be between 20-26 Degree Celcius with an ideal starting humidity levels of 50-55%. Below the temperature indicated, the vapours may condensate affecting the results of the operation.

How can we be certain that the decontamination cycle is completed? vapour cycles are based on the volume of the area to decontaminate. An indicator of the success of the operation is the percentage value of humidity built in the air during the vapourisation. When the readings on the humidity sensors reach values around 80-85%, it indicates that the concentration of H2O2 has reached its maximum, providing full diffusion of the vapours in the area.

How do the Biological and Chemical indicators work? Once the cycle is finished the indicators are collected and analysed. The success of the operation is usually guaranteed when a change in colour is observed in both Chemical and Biological Indicators. in this case study, the indicators supplied by Mesa labs changed colours from blue to pink for the Chemical indicators while the Biological indicators, after a period of incubation, showed 'No Growth' results by changing colour from yellow (the 'Positive' indicators not exposed to VPHP) to purple, therefore validating the operation.


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